Novel Isolation and Identification Methods for <i>Veillonella</i> Species Involved in Halitosis

Purpose: The purpose of the present study was to develop a novel selective medium (OVSM) and useful identification method for oral Veillonella species, assess prevalence this species using medium, investigate whether monitoring levels is as clinical indicator diagnosis halitosis. Methods: To examine bacterial populations in cavity, multiplex PCR were developed isolating identifying species. OVSM consists tryptic soy agar, sheep blood, sodium lactate, vancomycin, oxacillin. Results: Oral grew well on OVSM. accounted 5.4% 27.2%, respectively, all bacteria tongue biofilm samples healthy halitosis subjects. produced larger amounts volatile sulfur compounds than other representative bacteria. Conclusion: These results indicate that these organisms